DNA Nanotechnology: From Structure to Function

Free download. Book file PDF easily for everyone and every device. You can download and read online DNA Nanotechnology: From Structure to Function file PDF Book only if you are registered here. And also you can download or read online all Book PDF file that related with DNA Nanotechnology: From Structure to Function book. Happy reading DNA Nanotechnology: From Structure to Function Bookeveryone. Download file Free Book PDF DNA Nanotechnology: From Structure to Function at Complete PDF Library. This Book have some digital formats such us :paperbook, ebook, kindle, epub, fb2 and another formats. Here is The CompletePDF Book Library. It's free to register here to get Book file PDF DNA Nanotechnology: From Structure to Function Pocket Guide.
Structural DNA nanotechnology with programmed motions

New approaches in the production of error-free DNA exploit the use of self-assembly and natural error correction proteins. The enzyme is added to previously amplified PCR product, and this mixture is subjected to a second round of thermal cycling at the end of which it is put through gel electrophoresis, quantified, and cloned.

DNA Nanotechnology News, Articles | The Scientist Magazine®

It is, thus, most effective at removing common insertions and deletions that may occur during DNA synthesis [ 96 ]. In this case, DNA synthesis typically relies on spatial confinement of reactions to certain regions on a silica chip since this technology employs the addition of picoliters of reagents to the silica chip. Error rates can be reduced by controlling the locations on the chip where the reagents eventually end up. Another possibility could be directing reacting reagents through the use of photochemistry.

In this way, light can be used to block or restrict reactions at potential error sites. Directing redox reactions only at desirable sites in the forming DNA is another approach. All these strategies can help reduce error rates from 1 in bases to 1 in bases [ 98 ]. DNA is one for the most useful engineering materials available in nanotechnology. It has the potential for self-assembly and formation of programmable nanostructures, and it can also provide a platform for mechanical, chemical, and physical devices. While the formation of many complex nanoscale mechanisms has been perfected by nature over the course of millennia, scientists and engineers need to aggressively pursue the development of future technologies that can help expand the use of DNA in medicine, computation, material sciences, and physics.

It is imperative that nanotechnology is improved to meet the need for better detectors in the fields of biological and chemical detection and for higher sensitivity. In terms of DNA-based nanostructures, there is an urgent need to develop sophisticated architectures for diverse applications. Currently, much progress is being made in modelling DNA into various shapes through DNA origami, but the next step is to develop intelligent and refined structures that have viable physical, chemical, and biological applications.

Despite the fact that DNA computation may be in its infancy with limited forays into electronics and mathematics, future development of novel ways in which DNA would be utilized to have a much more comprehensive role in biological computation and data storage is envisaged. We are hopeful that the use of DNA molecules will eventually exceed expectations far beyond the scope of this review. His research interests are in the field of artificially designed DNA nanostructures and their applications in different fields, especially in biosensor applications, nanodevices designing and fabrication, and tissue engineering, especially in assisting burn patients.

Sekhon BS: Nanobiotechnology: an overview of drug discovery, delivery and development. Pharmacol Ther , Annu Rev Biochem , 65— Edited by: Bernstein M. Nature , — Nat Nanotechnol , 6 12 — Science , — Curr Opin Struct Biol , 20 3 — Seeman NC: Nucleic acid junctions and lattices. J Theor Biol , 99 2 — Angew Chem Int Ed , 46 1—2 — Chem Inform , 40 12 — New advances with filamentous phages. Eur Biophys J , 39 4 — Nano Lett , 5 4 — J Am Chem Soc , 50 — Angewandte Chemie , 28 — Org Biomol Chem , 4 18 — Angewandte Chemie , 44 — J Am Chem Soc , 34 — Angew Chem Int Ed , 49 49 — Angewandte Chemie , 17 — Nano Lett , 10 7 — Nat Biotechnol , 23 6 — Drug Discov Today , 13 1—2 — Int J Nanomedicine , 4: 1.

Clin Cancer Res , 11 11 — Biomaterials , 30 29 — Johnson JE: Virus particle maturation: insights into elegantly programmed nanomachines. Curr Opin Struct Biol , 20 2 — Merzlyak A, Lee S-W: Phage as templates for hybrid materials and mediators for nanomaterial synthesis. Curr Opin Chem Biol , 10 3 — Nat Mater , 6 8 — Curr Drug Deliv , 8 3 — Zou W: Immunosuppressive networks in the tumour environment and their therapeutic relevance.

Nat Rev Cancer , 5 4 — J Immunol , 6 — Nanotechnology, Science and Applications , 4: 1— Trends Anal Chem , — Trends Anal Chem , 14 6 — Curr Nanosci , 7 3 — Drug Resist Updat , 14 3 — Curr Appl Phys , 12 3 — J Am Chem Soc , 12 — ACS Nano , 3 9 — Nat Nanotechnol , 4 4 — Contraception , 67 2 — Contraception , 45 4 — Adleman LM: Molecular computation of solutions to combinatorial problems.

  • All Together in One Place (Kinship and Courage)?
  • DNA Nanotechnology for Cancer Therapy.
  • Search Results.
  • DNA nanotechnology: a future perspective.

J Comput Biol , 5 4 — DNA Comput , 70— Am J Mol Biol , 2 2 — Proc Natl Acad Sci , 97 4 — Nat Phys , 6 5 — London: Oxford University Press; New York: Wiley; Edited by: Kyoto RJ. Science Technica, Inc; — Biochem Cell Biol , 71 3—4 — J Mol Biol , 2 In Nanosystems Design and Technology. New York: Springer; — AIP Conference Proceedings , Phys Lett A , 3 — Anal Chim Acta , 1 — Thin Solid Films , — Phys Lett A , 6 — Curr Appl Phys , 9 1 :SS San Francisco; Appl Phys Lett , 97 19 ——3.

Appl Phys Lett , 96 2 ——3. Anal Bioanal Chem , 2 — J Electroanal Chem , 1 — Nano Lett , 4 12 — Appl Biochem Biotechnol , 5 — Nucleic Acids Res , 32 20 :ee Biotechnol Prog , 21 5 — Nucleic Acids Res , 31 6 :ee Proc Natl Acad Sci , 94 13 — EMBO Rep , 6 6 — Curr Protoc Mol Biol , 3 3.

Biochemistry , 39 13 — Nucleic Acids Res , 26 20 — Biotechniques , 44— Methods Enzymol , — Curr Opin Chem Biol , 16 3—4 — Nat Biotechnol , 28 12 — Download references. MZ and RA gathered the research data. All authors read and approved the final manuscript. Reprints and Permissions. Search all SpringerOpen articles Search. Abstract In addition to its genetic function, DNA is one of the most distinct and smart self-assembling nanomaterials.


Review Introduction Nucleic acids e. Figure 1. Full size image.

Figure 2. Sep 19, Do ESCs produce a large array of proteins? Sep 17, Related Stories. May 19, Oct 19, A better way to build DNA scaffolds May 06, Jul 24, Mar 28, Recommended for you. Nonviral gene therapy to speed up cancer research 3 hours ago. Electric tech could help reverse baldness Sep 19, Sep 18, User comments. Sign in.

Forgot Password Registration. What do you think about this particular story? Your message to the editors. Your email only if you want to be contacted back.

Hybrid, multiplexed, functional DNA nanotechnology for bioanalysis

Send Feedback. E-mail the story Non-aqueous solvent supports DNA nanotechnology. Your friend's email. Your email. I would like to subscribe to Science X Newsletter. Learn more. Your name. Note Your email address is used only to let the recipient know who sent the email. Your message. Your Privacy This site uses cookies to assist with navigation, analyse your use of our services, and provide content from third parties.

DNA nanotechnology

The protein particles were found to predominantly exist in monomeric form, while dimeric and multimeric forms were also observed both in free solution and bound to the quadruplex structure. The formation and the dissociation events of the G-quadruplexes were well documented in real-time and the intermediate-like states were also visualized. Here, we describe the direct visualization and single-molecule analysis of the formation of a tetramolecular G-quadruplex in KCl solution. The conformational changes were carried out by incorporating two duplex DNAs, with G—G mismatch repeats in the middle, inside a DNA origami frame and monitoring the topology change of the strands.

In the absence of KCl, incorporated duplexes had no interaction and laid parallel to each other. Addition of KCl induced the formation of a G-quadruplex structure by stably binding the duplexes to each other in the middle. Such a quadruplex formation allowed the DNA synapsis without disturbing the duplex regions of the participating sequences, and resulted in an X-shaped structure that was monitored by atomic force microscopy. Further, the G-quadruplex formation in KCl solution and its disruption in KCl-free buffer were analyzed in real-time.

Building 3D Structures with DNA Bricks

The orientation of the G-quadruplex is often difficult to control and investigate using traditional biochemical methods. However, our method using DNA origami could successfully control the strand orientations, topology and stoichiometry of the G-quadruplex. The B—Z DNA conformational transition requires the rotation of a double helix from a right-handed B-form to a left-handed Z-form structure. We herein designed a constrained and rotatable double-stranded DNA in which the rotational freedom was controlled by its placement into a DNA nanoscaffold. The Zab protein specifically binds to CG repeat sequences in Z-form double helices.

DNA Nanotechnology: From Structure to Function DNA Nanotechnology: From Structure to Function
DNA Nanotechnology: From Structure to Function DNA Nanotechnology: From Structure to Function
DNA Nanotechnology: From Structure to Function DNA Nanotechnology: From Structure to Function
DNA Nanotechnology: From Structure to Function DNA Nanotechnology: From Structure to Function
DNA Nanotechnology: From Structure to Function DNA Nanotechnology: From Structure to Function
DNA Nanotechnology: From Structure to Function DNA Nanotechnology: From Structure to Function
DNA Nanotechnology: From Structure to Function DNA Nanotechnology: From Structure to Function
DNA Nanotechnology: From Structure to Function DNA Nanotechnology: From Structure to Function

Related DNA Nanotechnology: From Structure to Function

Copyright 2019 - All Right Reserved